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Structured Review

Promega cell titer glow (promega)
Cell Titer Glow (Promega), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+titer+glow+(promega)/celltiter+glo+luminescent+cell+viability+assay/pm39454577-819-4-7
Average 90 stars, based on 1 article reviews
cell titer glow (promega) - by Bioz Stars, 2026-09
90/100 stars

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Article Snippet: Cell viability assays were conducted with cells irradiated for 4 min under identical conditions as used in other cell experiments after different additional cultivation times using the Cell-Titer Glow assay (Promega) according to the manufactures conditions.

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Article Snippet: We assessed cellular viability at the end-point of each experiment using Cell Titer-Glow (G7570, Promega), as per manufacturer’s instructions.

Magnetic Beads:

Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

Recombinant:

Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

Staining:

Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

Immunofluorescence:

Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

Blocking Assay:

Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

Protease Inhibitor:

Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

Western Blot:

Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

Sequencing:

Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

Transfection:

Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

Mutagenesis:

Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

Viability Assay:

Article Title: Agents and methods for treating dysproliferative diseases
Article Snippet: .. In a cell viability assay using cancer and normal cells lines MOLT-4 (human acute lymphoblastic leukemia [ALL]) and MRC-5 (human lung fibroblast), respectively, cell viability was assessed after 72 hours of exposure to a range of concentrations, using cell titer glow reagent from Promega to assess viability. ..



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Image Search Results


Journal: iScience

Article Title: Targeting ITGB4/SOX2-driven lung cancer stem cells using proteasome inhibitors

doi: 10.1016/j.isci.2023.107302

Figure Lengend Snippet:

Article Snippet: cell titer glow 3D assay , Promega , #G9681.

Techniques: Control, Virus, Recombinant, Live Cell Imaging, Chromatin Immunoprecipitation, Luciferase, CCK-8 Assay, Small Interfering RNA, shRNA, Software