cell titer glow (promega) (Promega)
90
Structured Review
Promega
cell titer glow (promega)
Cell Titer Glow (Promega), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+titer+glow+(promega)/celltiter+glo+luminescent+cell+viability+assay/pm39454577-819-4-7
Average 90 stars, based on 1 article reviews
Cell Titer Glow (Promega), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+titer+glow+(promega)/celltiter+glo+luminescent+cell+viability+assay/pm39454577-819-4-7
Average 90 stars, based on 1 article reviews
cell titer glow (promega) - by Bioz Stars,
2026-09
90/100 stars
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REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial Recombinant:Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial Staining:Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial Immunofluorescence:Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial Blocking Assay:Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial Protease Inhibitor:Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial Western Blot:Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial Sequencing:Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial Transfection:Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial Mutagenesis:Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial Viability Assay:Article Title: Agents and methods for treating dysproliferative diseases Article Snippet: .. In a cell viability assay using cancer and normal cells lines MOLT-4 (human acute lymphoblastic leukemia [ALL]) and MRC-5 (human lung fibroblast), respectively, cell viability was assessed after 72 hours of exposure to a range of concentrations, using |
